U18TR003778

Afs/Sers Saliva-Based Sars-Cov-2 Earliest Infection and Antibodies Detection - Project Summary/Abstract: This U18 Application is responsive to the NIH’s RADx-RAD emergency responses to the COVID- pandemic for new or non-traditional technologies developed for single extracellular vesicle, exosome and extracellular RNA (exRNA) isolation and analysis and reposition them for detection of SARS-CoV-2. The applicant’s group is a grantee in the NIH Common Fund “Extracellular RNA Communication (ERC)” program advancing a new and emerging technology of acoustofluidic separation (AFS) for label-free, high yield and purity exosomes from biofluids which is coupled to extracellular RNA characterization using surface enhanced Raman spectroscopy (SERS) for single EV identification. This U18 Application is to reposition the AFS EV technology and SERS for the non-invasive earliest detection of SARS-CoV-2 in saliva of infected patients. Host immunity to SARS-CoV-2 will also be assessed in the saliva samples, permitting the earliest detection of SARS-CoV-2 infection and host immunity non-invasively in a saliva sample. Five specific aims are in place to reposition the saliva-based AFS and SERS technologies, in a 2-year U18 proposal, to test the hypothesis that an integrated multi-parametric non-invasive saliva test for SARS-CoV-2 infection, viral load and host immunity test demonstrating clinical performance surpassing current saliva-based SARS-CoV-2 EUA tests.

Los Angeles University Of California

NOT APPLICABLE

93.360 - Biomedical Advanced Research and Development Authority (BARDA), Biodefense Medical Countermeasure Development

National Center for Advancing Translational Sciences (NCATS) [HHS - NIH]

National Institute of Allergy and Infectious Diseases (NIAID) [HHS - NIH]

Los Angeles, California 90095 United States

Single Zip Code

Emergency Awards: Exosome-based Non-traditional Technologies Towards Multi-Parametric and Integrated Approaches for SARS-CoV-2 (U18 Clinical Trial Not Allowed) (RFA-OD-20-018)

Amendment Since initial award the End Date has been extended from 11/30/22 to 11/30/23 and the total obligations have increased 99% from $904,071 to $1,796,802.