R03EB035329

Building a lightsheet system with dual-color fluorescence anisotropy capability for mapping protein-protein interactions in live cells - project summary: Protein-protein used combine becoming and designing for adapted used this imaging detector utilized different channel lightsheet pipeline handling fluorescence fluorescence anisotropy (or polarization) is routinely used for investigating interactions at high resolution in cellular environments. This imaging approach is in conjunction with TIRF, confocal or epi-illumination and lately there have been efforts to it with lightsheet.

Among the available fluorescence imaging modalities, lightsheet is a method of choice for 3D imaging of live samples because of its fast-imaging speed gentleness. We propose to leverage our experience in building lightsheet systems and polarization optics to advance the field of fluorescence polarization imaging. Our aim this proposal is to combine these two modalities in a manner such that it can easily be and used for biological applications and quantitative analysis.

Our previous research has a specifically designed Wollaston prism for imaging single color fluorescence anisotropy. In application, we propose to build a dual-color optical splitter for fluorescence polarization which will employ the Wollaston prism for polarization separation and a single area such as sCMOS camera (Aim 1a). Fabricated nanostructured pinhole arrays will be to characterize the optical splitter for field dependent distortions, transmission losses in channels (due to various optical components), and to validate the registration of multi- for polarization computation.

We will also combine this splitter with the home-built systems for 3D live imaging of cells and embryos (Aim 1b). A tailored image analysis along with a user-friendly graphical user interface (GUI) will be developed for data and quantitative image analysis (Aim 2). Our proposed approach would allow us to map anisotropy for investigating protein-protein interactions in 3D in live samples. C P

The Marine Biological Laboratory

TO SUPPORT HYPOTHESIS-, DESIGN-, TECHNOLOGY-, OR DEVICE-DRIVEN RESEARCH RELATED TO THE DISCOVERY, DESIGN, DEVELOPMENT, VALIDATION, AND APPLICATION OF TECHNOLOGIES FOR BIOMEDICAL IMAGING AND BIOENGINEERING. THE PROGRAM INCLUDES BIOMATERIALS (BIOMIMETICS, BIOPROCESSING, ORGANOGENESIS, REHABILITATION, TISSUE ENGINEERING, IMPLANT SCIENCE, MATERIAL SCIENCE, INTERFACE SCIENCE, PHYSICS AND STRESS ENGINEERING, TECHNOLOGY ASSESSMENT OF MATERIALS/DEVICES), BIOSENSORS/BIOTRANSDUCERS (TECHNOLOGY DEVELOPMENT, TECHNOLOGY ASSESSMENT, DEVELOPMENT OF ALGORITHMS, TELEMETRY), NANOTECHNOLOGY (NANOSCIENCE, BIOMIMETICS, DRUG DELIVERY SYSTEMS, DRUG BIOAVAILABILITY, MICROARRAY/COMBINATORIAL TECHNOLOGY, GENETIC ENGINEERING, COMPUTER SCIENCE, TECHNOLOGY ASSESSMENT), BIOINFORMATICS (COMPUTER SCIENCE, INFORMATION SCIENCE, MATHEMATICS, BIOMECHANICS, COMPUTATIONAL MODELING AND SIMULATION, REMOTE DIAGNOSIS AND THERAPY), IMAGING DEVICE DEVELOPMENT, BIOMEDICAL IMAGING TECHNOLOGY DEVELOPMENT, IMAGE EXPLOITATION, CONTRAST AGENTS, INFORMATICS AND COMPUTER SCIENCES RELATED TO IMAGING, MOLECULAR AND CELLULAR IMAGING, BIOELECTRICS/BIOMAGNETICS, ORGAN AND WHOLE BODY IMAGING, SCREENING FOR DISEASES AND DISORDERS, AND IMAGING TECHNOLOGY ASSESSMENT AND SURGERY (TECHNIQUE DEVELOPMENT AND TECHNOLOGY DEVELOPMENT).

93.286 - Discovery and Applied Research for Technological Innovations to Improve Human Health

National Institute of Biomedical Imaging and Bioengineering (NIBIB) [HHS - NIH]

Woods Hole, Massachusetts 025431015 United States

Single Zip Code

NIH Small Research Grant Program (Parent R03 Clinical Trial Not Allowed) (PA-20-200)

Amendment Since initial award the total obligations have increased 102% from $84,750 to $171,500.