R01MH129495

Extracellular Vesicles as Biomarkers of Trauma Exposure and PTSD Risk

Exposure to interpersonal violence (IPV) is associated with long-term negative mental health consequences, including an increased risk for posttraumatic stress disorder (PTSD), especially in women. The specific developmental timing of IPV is likely to play a significant role in disease outcomes, where trauma experienced in late adolescence increases PTSD risk by 3-fold. However, little is known about timing effects of IPV and PTSD risk related to sensitive periods of brain development and maturation.

Our previous studies examined IPV experienced during specific developmental windows on health outcomes in a predominantly black cohort of adult women. Our results revealed that psychophysiological reactivity related to PTSD risk, skin conductance response (SCR) and fear-potentiated startle, was uniquely observed when IPV was experienced between 14-18 years.

Using an unbiased proteomic approach in extracellular vesicles (EV) to identify potential biomarkers specific to timing of IPV experience, we found a unique EV protein signature belonging to a 17Q21 gene cluster and associated with skin keratinocytes, specifically unique to Merkel cells. Merkel cells are mechanosensitive neuroendocrine cells in the skin innervated by sensory AB neurons that detect light touch stimuli via PIEZO2 cation channels.

We found similar changes in a mouse model in which multimodal sensory stress was experienced during the pubertal period, including EV proteins associated with the mouse keratin I gene cluster 11QD, and increased adult fear-potentiated startle responses and freezing behaviors. Taken together, these results support tactile-based trauma exposure during late adolescence may increase sensitivity of threat circuitry.

The proposed cross-species translational study will examine molecular and physiological levels of analyses of the RDOC acute threat construct. In this proposal, we have the unique opportunity to investigate a 30-year prospective longitudinal black cohort who have been followed since birth, including collection of IPV data during late adolescence, and to capture their transition through young adulthood, providing insight into the mechanisms and biomarkers related to PTSD risk.

Our overarching hypothesis to be tested is that IPV occurring during a sensitive period of late adolescence specifically programs distinct biological pathways along the threat-response axis involving the skin Merkel cell-neurite complex, and that these changes are detectable as accessible biomarkers to be tested in the following aims:

1) To establish that a sensitive period of late adolescence for IPV experience uniquely associates with psychophysiological measures of acute threat, including SCR, fear-potentiated startle, and quantitative sensory testing in a prospective longitudinal birth cohort of black men and women;

2) To identify biomarkers from examination of EV proteomics and characteristics relevant to PTSD risk from a prospective longitudinal cohort; and

3) To identify the Merkel cell involvement in production of EVs and alteration of behaviors relevant to PTSD risk using pubertal multimodal sensory stress in a mouse.

The Regents Of The University Of Colorado

NOT APPLICABLE

93.242 - Mental Health Research Grants

National Institute of Mental Health (NIMH) [HHS - NIH]

Aurora, Colorado 800452507 United States

Single Zip Code

NIH Research Project Grant (Parent R01 Clinical Trial Not Allowed) (PA-20-185)

Amendment Since initial award the total obligations have increased 420% from $769,006 to $3,997,505.