R01ES033514

Environmental Chemical Body Burden and Prospective Breast Cancer Risk in the Cancer Prevention Study-3 Cohort

Today, there are more than 85,000 EPA-registered synthetic chemicals, but only 10% have been tested for carcinogenicity in animal studies. Of those tested, approximately 200 have shown evidence of causing cancer and/or an increase in mammary tumors. However, few of these chemicals have been evaluated in human studies, and the results in humans have been inconclusive.

Our study aims to focus on the chemical body burden of per- and poly-fluoroalkyl substances (PFAS) and the concomitant risk of developing invasive breast cancer during the menopausal transition. These "forever chemicals" are pervasive, enduring, and of high public interest. Yet, studies investigating their possible relationship to breast cancer have been limited.

We hypothesize that elevated body burden levels of these endocrine-disrupting chemicals (EDCs) will increase the risk of developing invasive breast cancer. We also propose that alterations in DNA methylation (DNAM) and the breast microenvironment are mechanisms that link these chemical exposures and breast cancer.

To test this hypothesis, we will employ a three-pronged approach. In Specific Aim 1, we will conduct a prospective study of women in the American Cancer Society Cancer Prevention Study-3 (CPS-3) to assess the association between the body burden of PFAS during the menopausal transition and the subsequent development of invasive breast cancer. We will measure PFAS levels in plasma samples collected 1 to 7 years before invasive breast cancer diagnosis in 1000 CPS-3 participants and 1000 matched, cancer-free CPS-3 participants, all between 40 and 57 years of age at blood draw.

In Specific Aim 2, using the same participants, we will measure DNAM using Infinium MethylationEPIC BeadChips and conduct an epigenome-wide association study (EWAS) to identify DNAM changes associated with levels of PFAS in the cancer-free participants. We will then determine the association between the PFAS-associated DNAM changes and the risk of developing breast cancer. This valuable DNAM data can also be used later for other outcomes, including exposure to other EDCs.

In Specific Aim 3, we will determine the direct effects of PFAS on tissue- and molecular-level states associated with susceptibility to cancer initiation in genomically well-characterized primary human breast mammary epithelial cells (HMECs). Identifying these mechanisms in primary breast cells is critically important as PFAS mechanisms of action generally differ by tissue. We will use 2-D cultures to determine the effects of short-term exposures and 3-D cultures to define the effects of protracted chemical exposures on changes in epithelial lineage consistent with accelerated aging and age-related molecular changes in genome methylation, lineage-specific transcription, and cytokeratin proteins.

Results from this multi-disciplinary approach will advance our understanding of the effects of PFAS exposures on the risk of developing breast cancer during an important window of susceptibility. Ultimately, we hope that the results from our proposed project will identify an integrated biological signature of environmental exposure, deliver mechanistic insights into breast cancer development from EDCs, and inform future studies for prevention strategies to reduce or mitigate exposures.

Beckman Research Institute Of The City Of Hope

THE MISSION OF THE NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES (NIEHS) IS TO RESEARCH HOW THE ENVIRONMENT AFFECTS BIOLOGICAL SYSTEMS ACROSS THE LIFESPAN AND TO TRANSLATE THIS KNOWLEDGE TO REDUCE DISEASE AND PROMOTE HEALTH.

93.113 - Environmental Health

National Institute of Environmental Health Sciences (NIEHS) [HHS - NIH]

Duarte, California 910103012 United States

Single Zip Code

NIH Research Project Grant (Parent R01 Clinical Trial Not Allowed) (PA-20-185)

Amendment Since initial award the total obligations have increased 386% from $649,634 to $3,158,162.