DP1EB033154

Sonogenetic Remote Control of Cellular Function - Summary

The discovery and development of fluorescent proteins and optogenetics revolutionized biology by making it possible to image and control specific cellular processes with visible light. While these tools have enabled countless biological discoveries, the poor penetration of light into living tissue makes it difficult to use optical techniques in intact animals. As a result, biological phenomena ranging from the signaling of neurons in deep-brain regions to the infiltration of immune cells into tumors to the microbial colonization of the GI tract are challenging to study within their natural in vivo context.

If instead of light, it were possible to visualize and manipulate cellular function using a more penetrant form of energy such as ultrasound, this would open previously inaccessible domains of in vivo biology to direct investigation. In addition, it would enhance the development of cell-based therapies by allowing cellular agents to be seen and controlled after administration into the human body.

The physics of ultrasound make it an ideal modality for deep-tissue cellular communication. Sound waves in the MHz range are weakly scattered by tissue and can therefore penetrate several cm into the body. With wavelengths on the order of 100 μm and travel times < 1 ms, ultrasound can access many key structures and processes. When focused, sound waves can deliver mechanical and thermal energy to precise anatomical locations. These properties have already made ultrasound one of the world's most widely used technologies for medical imaging and non-invasive surgery. However, the potential of ultrasound to serve as a tool for cellular imaging and control has been relatively untapped due to a lack of methods to connect it to the function of specific cells and biomolecules.

In previous work, the Shapiro Lab has pioneered the use of ultrasound in cellular and molecular imaging by developing the first acoustic reporter genes and biosensors for ultrasound, aiming to "do for ultrasound what fluorescent proteins have done for fluorescence microscopy". The major goal of our proposed new research direction is to "do for ultrasound what optogenetics has done for light" by giving sound waves the ability to control specific cellular functions such as neuronal excitation, gene expression, and intracellular signaling in vivo.

The basic principle of our approach is to (1) use focused ultrasound to deposit acoustic energy at a specific location in tissue, (2) use genetically encoded "acoustic antennae" to convert this energy into local mechanical force, and (3) use this force to actuate mechanosensitive receptors to produce specific cellular signals. We will implement this approach in neurons and immune cells to enable unique neuroscience and cell therapy applications. If successful, this work will help establish the new field of sonogenetics by providing researchers and clinicians with the unprecedented ability to "point and click" on cells deep within the body and tell them what to do.

California Institute Of Technology

NOT APPLICABLE

93.310 - Trans-NIH Research Support

National Institute of Biomedical Imaging and Bioengineering (NIBIB) [HHS - NIH]

National Institute of Allergy and Infectious Diseases (NIAID) [HHS - NIH]

Pasadena, California 911250001 United States

Single Zip Code

NIH Directors Pioneer Award Program (DP1 Clinical Trial Optional) (RFA-RM-20-011)

Amendment Since initial award the total obligations have increased 400% from $1,172,500 to $5,862,500.