Single-Nucleus Multiome Reagents (Brand Name 10X Genomics or Equal)

Background
The USDA Agricultural Research Service (ARS) Dairy Forage Research Center (DFRC) in Madison, Wisconsin, is seeking to procure reagents for single-cell or single-nucleus profiling across cattle samples. This initiative aims to enhance germplasm conservation, functional genomics, and national livestock improvement programs by characterizing gene regulation, chromatin accessibility, and adaptive immune diversity in cattle. The solicitation number for this request is 1232SA26Q0504.

Work Details
The contractor must provide the following items:

A. For single-nucleus Multiome workflow for 36 samples:
1 x Single Index Kit N Set A (Brand Name 10X Genomics 1000212 or Equal)
1 x GEM-X Epi Multiome, 4 samples (Brand Name 10X Genomics 1000980 or Equal)
2 x GEM-X Epi Multiome, 16 samples (Brand Name 10X Genomics 1000979 or Equal)
3 x GEM-X Epi Multiome Chip Kit, 4 chips (Brand Name 10X Genomics 1000951 or Equal)

B. For single-cell microbiome RNAseq adaptable to BacDrop bacterial applications:
2 x Chromium Next GEM Single Cell ATAC Kit v2, 16 rxns (Brand Name 10X Genomics 1000390 or Equal)
2 x Chromium Next GEM Chip H Single Cell Kit, 48 rxns (Brand Name 10X Genomics 1000161 or Equal)

C. For 5’ single-cell gene expression with BCR/TCR repertoire enrichment (n=48):
3 x GEM-X Universal 5' Gene Expression v3 4-plex (16 samples) (Brand Name 10X Genomics 1000780 or Equal)
3 x GEM-X OCM 5' Chip Kit v3 4-plex, 2 chips (Brand Name 10X Genomics 1000748 or Equal)
1 x Dual Index Kit TT Set A, 96 rxn (Brand Name 10X Genomics 1000215 or Equal).

All items must have clear traceability to the Original Equipment Manufacturer (OEM), specifically the brand name of '10X Genomics', ensuring continuity of research and data integrity.

Period of Performance
Delivery is required by September 30, 2026.

Place of Performance
The delivery location is the USDA ARS Dairy Forage Research Center at 1925 Linden Drive, Madison, Wisconsin.

Bidder Requirements
Offerors must provide a Certificate of Conformance and demonstrate traceability back to the manufacturer or authorized distributor. Vendors not being the OEM must provide documentation proving their products meet all salient characteristics outlined in the solicitation.

Incumbent Analysis (see Incumbents section for more detail)
The solicitation for Single-Nucleus Multiome reagents (10X Genomics or equal) shows a closely related prior contract, 1232SA25P0316, awarded to 10X Genomics for Chromium X and associated single-cell partitioning and sequencing library preparation kits valued at $75,720 (09/08/25–12/01/25). The prior work appears to align with the current requirement for 10X Genomics Multiome/Chromium-based reagent kits and accessories used to generate single-cell/single-nucleus sequencing libraries, suggesting continuity in the agency’s ongoing single-cell workflow needs.