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RNA Extraction and Sequencing   2

ID: 1232SA25Q0142 • Type: Synopsis Solicitation • Match:  85%
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Description

04JUN25 Base posting. Any amendment and document updates related to this solicitation will be available electronically at the Government Point of Entry (sam.gov). It is the offeror's responsibility to monitor sam.gov Contract Opportunities regarding this solicitation.
(i)This is a combined synopsis/solicitation for a commercial service prepared in accordance with the format in FAR subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued.
(ii)The solicitation number is 1232SA25Q0142 and is issued as a request for quotation (RFQ).
(iii)This solicitation document incorporates those provisions and clauses in effect through Federal Acquisition Circular 2025-03.
(iv)Set Aside: Total Small Business. NAICS Code: 541380 Small Business Size Standard: $19 million
(v) See clauses and provisions document section SCHEDULE and CLIN Structure for line item number(s), quantity, and unit of measure.
(vi)The USDA, Agricultural Research Service requires RNA Extraction and Sequencing. See Attachment 001 Performance Work Statement and QASP and other attachments for more information.
(vii)Dates: See clauses and provisions document GENERAL INFORMATION section for Period of Performance data
Place of Delivery and Acceptance:
NEA COOL AND COLD WATER AQUACULTURE
11861 LEETOWN ROAD
KEARNEYSVILLE WV 25430
FOB Point: Destination
(viii)The provision at 52.212-1, Instructions to Offerors-Commercial Products and Commercial Services, applies to this acquisition. See Instructions for the Preparation of Offers (FAR 52.212-1 Addendum) in the clauses and provisions document.
(ix)The provision at 52.212-2, Evaluation-Commercial Products and Commercial Services is detailed in the clauses and provisions document under FAR 52.212-2 Evaluation-Commercial Items (Addendum.)
(x)Online sam.gov completion of the provision at 52.212-3 is advised.
(xi)The clause at 52.212-4, Contract Terms and Conditions-Commercial Products and Commercial Services, applies to this acquisition. Any addenda will be noted in the clauses and provisions document.
(xii)The clause at 52.212-5, Contract Terms and Conditions Required To Implement Statutes or Executive Orders-Commercial Products and Commercial Services, applies to this acquisition. Additional clauses are detailed in the clauses and provisions document.
(xiii)Not applicable.
(xiv)Not applicable.
(xv)All sources wishing to quote shall furnish a quotation by the time and dated noted in the General Information, Date Offers Due fields above.
(xvi)Quotations are to be sent via email to Christopher Turner at christopher.c.turner@usda.gov.
Notes:
Solicitation Inquiries:
a. Inquiries and all correspondence concerning this solicitation prior to contract award shall be submitted in writing only to the Contracting Officer.
b. Inquiries shall be via Email only, no phone inquiries will be accepted.
c. The Contracting Officer's email contact information is listed as the PRIMARY POINT OF CONTACT in the sam.gov Contract Opportunities posting.
Time Zones: Please note time zone designations in the "General Information" section above. Other time zones are as designated in the solicitation attachments.
Failure to provide the required information in the requested format may result in quotes not being considered for award.
Attached Provisions/Clauses apply to this RFQ and any subsequent award.

Background
The USDA Agricultural Research Service (ARS) is seeking to procure RNA extraction and sequencing services for research purposes. The goal of this contract is to facilitate the analysis of rainbow trout samples, which are essential for advancing agricultural research and understanding aquatic ecosystems.

Work Details
The contractor will perform total RNA extraction and mRNA sequencing on 120 frozen samples of rainbow trout whole-body tissue homogenized in Trizol. The contractor must provide all necessary services, supplies, equipment, and facilities. Specific tasks include:

1. Extracting and quantifying total RNA, ensuring a minimum concentration of >250ng/uL and a minimum volume of >200uL stored in nuclease-free water.

2. Re-extracting total RNA until quality standards are met, with A260/A280 values between 1.8 to 2.0 and RIN values greater than 7 as evaluated by BioAnalyzer or similar technology.

3. Performing standard Illumina RNA-seq library preparation using Illumina technology with paired-end reads of 2 x 150 nucleotides.

4. Delivering a minimum of 40 million paired-end reads per sample with over 85% of bases having QC≥30.

5. Implementing quality control steps during RNA extraction, library preparation, and sequencing.

6. Returning remaining total RNA in labeled microcentrifuge tubes shipped overnight with dry ice.

7. Providing RNA sequencing data for all samples along with a Final Project Report detailing the processes undertaken.

Period of Performance
The contract will be performed over a period of approximately 90 days after receipt of order (ARO), with specific deliverables due within set timeframes following the award.

Place of Performance
The work will be performed at the NEA Cool and Cold Water Aquaculture facility located at 11861 Leetown Road, Kearneysville, WV 25430.

Overview

Response Deadline
June 15, 2025, 9:00 a.m. EDT Past Due
Posted
June 4, 2025, 12:48 p.m. EDT
Set Aside
Small Business (SBA)
Place of Performance
Kearneysville, WV 25430 United States
Source

Current SBA Size Standard
$19 Million
Pricing
Likely Fixed Price
Est. Level of Competition
Low
Est. Value Range
$19,000,000 (value based on agency estimated range)
Signs of Shaping
69% of obligations for similar contracts within the Agricultural Research Service were awarded full & open.
On 6/4/25 Agricultural Research Service issued Synopsis Solicitation 1232SA25Q0142 for RNA Extraction and Sequencing due 6/15/25. The opportunity was issued with a Small Business (SBA) set aside with NAICS 541380 (SBA Size Standard $19 Million) and PSC B529.
Primary Contact
Name
Christopher Turner   Profile
Phone
None

Documents

Posted documents for Synopsis Solicitation 1232SA25Q0142

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Additional Details

Source Agency Hierarchy
AGRICULTURE, DEPARTMENT OF > AGRICULTURAL RESEARCH SERVICE > USDA ARS AFM APD
FPDS Organization Code
12H2-1232SA
Source Organization Code
500022476
Last Updated
June 30, 2025
Last Updated By
chris.c.turner@usda.gov
Archive Date
June 30, 2025