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Real-Time Quantitative Polymerase Chain Reaction (qPCR) Machine (Brand Name CFX Opus 96 or Equal)   2

ID: 1232SA25Q0864 • Type: Synopsis Solicitation • Match:  85%
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Description

This is a combined synopsis/solicitation for commercial item prepared in accordance with the format in FAR subpart 12.6 and FAR Part 13, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested, and a written solicitation will not be issued. The solicitation number is 1232SA25Q0864 and is issued as a request for quotation (RFQ). This solicitation document incorporates those provisions and clauses in effect through Federal Acquisition Circular 2025-05. The associated NAICS Code is 334516 and the Small Business Size Standard is 1,000 employees.
The USDA, Agricultural Research Service, requires Real-Time Quantitative Polymerase Chain Reaction (qPCR) Machine (Brand Name CFX Opus 96 or Equal) for USDA ARS Plant Science Research Unit in St Paul, MN. The Government intends to award a single firm-fixed price purchase order.
The requirements are further detailed in the attached Requirements Document.
The DELIVERY ADDRESS is:
USDA-ARS, Plant Science Research Unit
1991 Upper Buford Circle, Rm 411
Borlaug Hall., St. Paul, MN 55108

DOCUMENTS TO BE INCLUDED IN QUOTE: In order to be considered for award, Offerors shall respond by:
1. Providing a quote on company letterhead addressing all documents identified in the RFQ according to requirements as defined in section FAR 52.212-1 Addendum Submission documents, clearly note per item pricing, with all associated costs to include but not limited to shipping/handling/delivery price, and be valid for at least 60 days after receipt of quote.
2. Any amendment and documents related to this procurement will be available electronically at the Government Point of Entry (sam.gov).
FAR clauses 52.211-6, 52.212-1, 52.212-2, 52.212-3, 52.212-4 and 52.212-5 (and any addenda to those clauses) are included in the attached clauses and provisions document. Additionally, other contract requirements and terms/conditions can be located in the same document.
The Government anticipates award of a contract resulting from this solicitation to the responsible offerors whose quote conforms to the specifications that will be the most advantageous to the Government in accordance with FAR 13.106-2 considering three major areas: technical acceptance, past performance and price. Quotes will be evaluated by performing a comparative evaluation in accordance with FAR 13.106-2(b)(3) to determine which quote meets the lowest price technically acceptable evaluation methodology.
All sources wishing to quote shall furnish a quotation on or before the date identified on the solicitation document. Quotations are to be sent via email to Shane Pope at shane.pope@usda.gov .
Failure to provide the required information in the requested format may result in quotes not being considered for award.
Attached Provisions/Clauses apply to this RFQ and any subsequent award.

Background
The United States Department of Agriculture (USDA), Agricultural Research Service (ARS) is seeking to procure a Real-Time Quantitative Polymerase Chain Reaction (qPCR) Machine, specifically the Brand Name CFX Opus 96 or an equivalent model. This procurement is aimed at enhancing research capabilities at the USDA ARS Plant Science Research Unit located in St. Paul, Minnesota. The research focuses on improving agronomic traits in crops such as alfalfa, including root architecture, winter survival, disease resistance, and carbon sequestration in soil. The qPCR machine will facilitate the assessment of functional genes in various experiments.

Work Details
The contract requires the delivery of the following items:

1. **Real-Time Quantitative Polymerase Chain Reaction (qPCR) Machine** (Brand Name CFX Opus 96 or Equal)
- Quantity: 1 EA
- Specifications:
- At least 96-well capacity
- Minimum 5-color plus FRET capability
- Network connected real-time PCR detection system
- Price must be all-inclusive, covering delivery and applicable fees

2. **Wi-Fi Adapter** (Brand Name CFX or Equal)
- Quantity: 1 EA
- Specifications:
- Enables Wi-Fi connection for North America and other specific countries.

3. **Hard-Shell PCR Plates** (Brand Name CFX or Equal)
- Quantity: 1 EA
- Specifications:
- At least 96-Well PCR Plates with low profile thin wall, skirted, white/clear Pkg of 50.

4. **Microseal 'B' PCR Plate Sealing Film** (Brand Name CFX or Equal)
- Quantity: 1 EA
- Specifications:
- Adhesive, optical Pkg of 100; optically clear seal for PCR plates.

5. **Advanced cDNA Synthesis Kit** (Brand Name iScrip or Equal)
- Quantity: 1 EA
- Specifications:
- No less than 25 x 20 µl reactions; must include 100 µl Advanced 5x Reaction Mix and 25 µl Advanced Reverse Transcriptase.

6. **Green Supermix** (Brand Name SsoAdvanced Universal SYBR or Equal)
- Quantity: 1 EA
- Specifications:
- For 200 x 20 µl reactions, packaged in two vials of 1 ml each; must contain dNTPs and Sso7d fusion polymerase.

7. **Probes Supermix** (Brand Name SsoAdvanced Universal or Equal)
- Quantity: 1 EA
- Specifications:
- For 200 x 20 µl reactions, packaged in two vials of 1 ml each; contains dNTPs and stabilizers.

Period of Performance
Delivery is required within 30 days after award.

Place of Performance
The products will be delivered to USDA-ARS, Plant Science Research Unit, located at 1991 Upper Buford Circle, Rm 411, Borlaug Hall., St. Paul, MN 55108.

Overview

Response Deadline
Sept. 16, 2025, 6:00 p.m. EDT Past Due
Posted
Sept. 11, 2025, 6:00 p.m. EDT
Set Aside
Small Business (SBA)
Place of Performance
Saint Paul, MN 55108 United States
Source

Current SBA Size Standard
1000 Employees
Pricing
Fixed Price
Evaluation Criteria
Lowest Price Technically Acceptable (LPTA)
Est. Level of Competition
Low
Signs of Shaping
The solicitation is open for 4 days, below average for the Agricultural Research Service. 81% of obligations for similar contracts within the Agricultural Research Service were awarded full & open.
On 9/11/25 Agricultural Research Service issued Synopsis Solicitation 1232SA25Q0864 for Real-Time Quantitative Polymerase Chain Reaction (qPCR) Machine (Brand Name CFX Opus 96 or Equal) due 9/16/25. The opportunity was issued with a Small Business (SBA) set aside with NAICS 334516 (SBA Size Standard 1000 Employees) and PSC 6640.
Primary Contact
Name
Shane Pope   Profile
Phone
(970) 851-3735

Documents

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Additional Details

Source Agency Hierarchy
AGRICULTURE, DEPARTMENT OF > AGRICULTURAL RESEARCH SERVICE > USDA ARS AFM APD
FPDS Organization Code
12H2-1232SA
Source Organization Code
500022476
Last Updated
Sept. 11, 2025
Last Updated By
shane.pope@usda.gov
Archive Date
Oct. 1, 2025