Sequencing 389 TNA Samples

Background
The U.S. Environmental Protection Agency (EPA) has a requirement for a sole source fixed-priced Purchase Order to sequence 389 TNA samples and prepare a custom processing library. The University of California, Davis Genome Center is identified as the only qualified entity to provide these specialized services, which are critical for viral discovery.

Work Details
The contract involves the following tasks:
1. Provide a single, custom processing library preparation method for sequencing both RNA and DNA from 389 samples.
2. Utilize a proprietary methodology to sequence both DNA and RNA simultaneously without targets, ensuring unbiased sequencing essential for viral discovery.
3. Ensure that new data generated aligns seamlessly with past results provided to the EPA by the same facility, maintaining continuity and compatibility in data sets.
4. Leverage unique operational nuances of UC Davis DNA Technologies and Expression Analysis Core that contribute to high-quality data reliability and integrity.

Place of Performance
The services will be performed at the University of California, Davis Genome Center located at One Shields Ave, Davis, CA 95616.

Bidder Requirements
Only the University of California, Davis Genome Center can furnish the required services due to their proprietary methodologies and previous experience with EPA projects.

Incumbent Analysis (see Incumbents section for more detail)
The prior contract 68HE0B25P0392 awarded to Davis University of California (value $55,000; 04/21/25–12/31/25) for sequencing 300 TNA samples and preparing libraries appears closely related to this requirement to sequence 389 TNA samples and prepare a custom processing library. The earlier work’s focus on TNA sample sequencing and library preparation, along with the current emphasis on producing data that aligns with past results, suggests this effort may be a continuation or follow-on expansion of the same sequencing/library workflow.