NovaSeq DNA sequencing of blue whales for PMEL Omics

Background
The PMEL Omics group is seeking a contract for NovaSeq sequencing of blue whale genomic data and associated DNA sequencing prep work. The contractor must have an Illumina NovaSeq S4 2x150bp flow cell sequencer and the capabilities to work with non-model species and varying quality of input DNA to perform whole genome re-sequencing. The samples are very limited in quantity and irreplaceable, so the facility needs to maintain best practices and a sterile environment to limit the potential for cross-contamination with other projects.
The lab is required to have a track record of working with non-model organisms and the ability to adjust the sequencing scheme based on the data output and standard sequencing quality scores after each run. They must communicate with the PMEL Omic group throughout the project to ensure high-quality data is produced.

Work Details
The scope of work includes NovaSeq DNA sequencing of blue whales for PMEL Omics, which involves library preparation (barcoding, normalization, and general QC) for up to 90 samples. The samples will be pooled, sequenced, and checked for coverage/depth after each run on a NovaSeq lane. Samples may arrive in 2 batches depending on availability and international shipping. The deliverables include whole genome resequencing at 10-15X depth coverage of approximately 90 blue whale individuals, with data to be delivered within 90 days of receipt of the samples. Data will be demultiplexed by barcoded individual prior to returning the data, and quality scores of sequences will be evaluated between each lane of sequencing to determine the best course of action.