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DNA Isolation Reagents

ID: 12405B23Q0493 • Type: Solicitation
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Description

Statement of work
The United States Department of Agriculture, Agricultural Research Service, Plant Science Research Unit, Raleigh, North Carolina requires:
Acquisition of laboratory consumables for extracting DNA from large populations of plants to facilitate screening of the plants using marker and genomic assisted selection and sequencing.
The LGC oKtopure automated DNA extraction instrument is used to isolate 800-1600 DNA samples/day from plat tissue for high-throughput screening using PCR based markers to provide data to plant breeders who have submitted the samples.
These reagents, tips and 96-well magnet plates are key to maintaining sample integrity without risk of contamination and to meet instrument requirements.
Requirements:
1. Customized sbeadex magnetic bead based plant DNA extraction kits specifically designed for use on the LGC oKtopure automated liquid hadling instrument and optimized for the ERSGGL (Eastern Regional Small Grains Genotyping Lab) wheat & barley extraction requirements.
2. AMP Elution Buffer base reagent for DNA concentration quantitation with PicoGreen
3. 200ul robotic pipetting tips that meet the specified requirements of the instrument and the reagent plates.
4. 96-well storage plates are calibrated to the magnets on the instrument and the reagent volumes required for washing the DNA samples.
5. Freight must be included to destination
Acceptable Reagents:
NAP41615 sbeadex maxi plant oKtopure *customized USDA ARS NC with additional AMP buffer, MagMix 96-well storage plates and robotic tips.

Background
The United States Department of Agriculture, Agricultural Research Service, Plant Science Research Unit in Raleigh, North Carolina requires the acquisition of laboratory consumables for extracting DNA from large populations of plants. The LGC oKtopure automated DNA extraction instrument is used to isolate 800-1600 DNA samples/day from plant tissue for high-throughput screening using PCR based markers to provide data to plant breeders who have submitted the samples. The reagents, tips, and 96-well magnet plates are key to maintaining sample integrity without risk of contamination and to meet instrument requirements.

Work Details
1. Customized sbeadex magnetic bead based plant DNA extraction kits specifically designed for use on the LGC oKtopure automated liquid handling instrument and optimized for the ERSGGL (Eastern Regional Small Grains Genotyping Lab) wheat & barley extraction requirements.
2. AMP Elution Buffer base reagent for DNA concentration quantitation with PicoGreen.
3. 200ul robotic pipetting tips that meet the specified requirements of the instrument and the reagent plates.
4. 96-well storage plates are calibrated to the magnets on the instrument and the reagent volumes required for washing the DNA samples.
5. Freight must be included to destination.
Acceptable Reagents: NAP41615 sbeadex maxi plant oKtopure *customized USDA ARS NC with additional AMP buffer, MagMix 96-well storage plates and robotic tips.

Place of Performance
USDA, ARS, SEA, 840 Oval Dr., 2105 NC State Plant, Raleigh, NC 27606
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Overview

Agency
ARS Southeast Area (SEA) [USDA - ARS]
Response Deadline
Aug. 18, 2023, 5:00 p.m. EDT Past Due
Posted
Aug. 14, 2023, 10:47 a.m. EDT
Set Aside
None
NAICS
541714 - Research and Development in Biotechnology (except Nanobiotechnology)
PSC
6640 - Laboratory Equipment And Supplies
Place of Performance
Raleigh, NC 27606 United States
Source
SAM
Current SBA Size Standard
1000 Employees
Pricing
Likely Fixed Price
Evaluation Criteria
Lowest Price Technically Acceptable (LPTA)
Est. Level of Competition
Average
Signs of Shaping
The solicitation is open for 4 days, below average for the ARS Southeast Area.
On 8/14/23 ARS Southeast Area issued Solicitation 12405B23Q0493 for DNA Isolation Reagents due 8/18/23. The opportunity was issued full & open with NAICS 541714 and PSC 6640.
Primary Contact
Name
Cecilia McNamee   Profile
Email
cecilia.mcnamee@usda.gov
Phone
(985) 232-8289

Documents

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Additional Details

Source Agency Hierarchy
AGRICULTURE, DEPARTMENT OF > AGRICULTURAL RESEARCH SERVICE > USDA ARS SEA AAO ACQ/PER PROP
FPDS Organization Code
12H2-12405B
Source Organization Code
500033339
Last Updated
Sept. 2, 2023
Last Updated By
cecilia.mcnamee@usda.gov
Archive Date
Sept. 2, 2023